The deoxyribonucleic acid(DNA) was isolated from the pulp, dentin and enamel of the 4 fresh teeth and the 7 teeth left in room temperature for 10 years. Then it was examed to find out the usefulness for forensic dental medicine.
Samples of the tooth-derived DNA amplified by polymerase chain reaction(PCR), electrophosed for sex determination by detection of X-Y homologous amelogenin gene and DIS80 locus detection.
@ES The following results have been achieved.
@EN 1. DNA extraction was possible in pulp and dentin of the fresh teeth, so it could be applicatable to detection of X-Y homologous amelogenin gene for sex determination, amplification of DIS80 locus by PCR.
2. Sex determination was possible in pulp and dentin of the teeth left at room temperature for 10 years. Also, possible the detection of AMP-FLPs to increase PCR cycling up to 40.
3. DNA was isolated from all pulp of the fresh teeth and the teeth left in room temperature for 10 years, and also isolated from the dentin of the fresh teeth, partially isolated(3/7) from the dentin of the teeth left in room temperature for 10
years,
but DNA was not isolated from the enamel.
From the above investigation, DNA extraction, sex determination, amplification of DIS80 locus were successfully accomplished even though the teeth were left for 10 years at room temperature. Therefore, teeth, especially pulp, are highly reliable
and
applicatable as molecular biological samples for individual identification.
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